Isolation and identification of cellulolytic bacteria from Waste organic vegetables and fruits for role in making Materials biogas.

Supriatna, Ateng and Rohimah, Ida and Suryani, Yani and Sa'adah, Sumiyati Isolation and identification of cellulolytic bacteria from Waste organic vegetables and fruits for role in making Materials biogas. Istek. ISSN 1979-8911

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Abstract

Research on isolation and identificationof cellulolytic bacteria from waste organic vegetables and fruitsas ingredients that play a rolein the manufacture of biogas has been carried out. Bacteria isolates were obtained from waste vegetable and fruits from the market Gedebage Bandung. The aim of research is to know diversity of bacteria that grow and cellulolytic bacterial isolates whatever ison the waste vegetables and fruit sthat play a rolein making biogas. Media that used for cultivation and isolation bacteria is NA (Natrium Agar). Bacteria isolation is conducted by using serial dilution, pour plate and streak plate methods. The determine cellulolytic bacteria used selective media SA(Cellulose Agar) and identification ofbacteriacarriedto genus level based on morphological and biochemical characterization that refersto the guidelines of identification of bacteria (Bergey's Manual of Determinative Bacteriology in 1994). In this research, we successfully isolated 13 isolates bacteria that consist of 10 genera. Identification result indicates that isolate B1, B8, B9, and B12 are Bacillus; isolate B2 is Cellulomonas, isolate B4 is Microbacterium, isolate B5 is Neisseria, isolate B6 is Streptococcus and isolate B7 is Strepmycetes. Test cellulose indicates that the cellulolytic bacteria are Bacillus, Cellulomonas, Microbacterium, Streptomycetes, Streptococcus, and Neisseria. Based on test in HC degradation of cellulose in the mediaSA, Streptomycetesis bacteria that have the highest HC ratio and the lowest in the genus Neisseria. The bacteria were chosen because the bacteria have a high ability to hydrolyze cellulose inso called cellulolytic bacteria and potentiallyin the manufacture of biogas.

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